qEV100 / 35nm - 1 Pack

Exosome isolation for 100ml samples - Range 35 - 350 nm

Capable of running 100 mL samples, the qEV100 is ideal for any application that requires large sample volumes, whether it’s the output of bioreactors, dilute cell culture supernatants or materials with high protein levels like plasma or serum. Many of these biological fluids cannot be concentrated further due to the propensity for protein complex formation and vesicle aggregation, which can destroy biological function. Like all of Izon’s qEV columns, the qEV100 provides a gentle, rapid method for maximizing recovery of biological particles. No ultracentrifugation. No organic solvents.  

The qEV100 is also available with the 70 nm+ Isolation range

Videos

How qEV / Size Exclusion Chromatography Works

Technical Features

Specifications

Column Name

qEV100

qEV100

Column series

qEV100 / 70

qEV100 / 35

Optimal Separation Size

70 - 1000 nm

35 - 350 nm

Nominal flow rate (mL/min at 20°C)

15

11

Column Volume (mL)

750

750

Sample Load Volume (mL)

Up to 100mL

Up to 100mL

Optimal fraction size (mL)

25 or 50

25 or 50

Void volume (mL)

150

150

Minimum flush volume (mL)

700

700

Nominal peak elution volume (mL)

200

200

Elution peak after void (mL)

75 ± 25

75 ± 25

Operational temperature

18 to 24 °C

18 to 24 °C

Buffer

PBS

PBS

Largest size passable

1 µm

1 µm

Top and bottom filters size

20 µm

20 µm

pH stability working range

3 - 13

3 - 13

pH stability cleaning-in-range (CIP)

2 - 14

2 - 14

Shelf life (if stored correctly)

12 months

12 months

Application Fields