FluidFM BOT CRISPR

Deliver your CRISPR-Cas complexes directly to where they are needed: the nucleus

Designed for pharmacologists and molecular biologists, the FluidFM CRISPR is the most reliable and cell-friendly solution to edit the genome of adherent cells.

Unlike traditional transfection methods, benefit from requiring only a small number of starting cells thanks to high-viability and transfection efficiency even on notably hard-to-transfect cell types. 

With the CRISPR system, pass easily over the cytoplasmic and nuclear membranes in directly delivering the material to the nucleus, making sure that your CRISPR-Cas complexes and repair template reach their targets. This increases the chance of HDR events to happen.

With the integrated Volume Calculator feature, calculate precisely the number of delivered complexes and optimize the dosage to maximize on target and minimize off target effects.

Perform multiplex editing – deliver as many gRNA as you want in a single nucleus, in a single injection.

Product Highlights
  • Selective, direct & gentle intra-cellular injection
    Precisely select single cells based on their phenotype and deliver with the tiny FluidFM nanosyringe (aperture 600 nm) CRISPR-Cas complexes exactly where you need them – the nucleus. The cell’s membrane instantaneously reseals when the nanosyringe is retracted, resulting in cell viability of over 95%.
  • Intuitive & easy
    Benefit from simple preparation and extensive automation and data management combined with our user-friendly and intuitive software. Simply point and click on the cytoplasm or nucleus of the cells you want to target and click "OK". It is as straightforward as that.
  • Volume calculator
    Calculate precisely the number of delivered complexes with the integrated Volume Calculator feature. This is particularly useful when you want to optimize the dosage to maximize on target and minimize off target effects.
  • Integrated fluorescence microscopy
    • Fluorescence light source: 130 W Hg light guide illumination
    • Motorized turret with 8 positions, built-in shutter, simple waterproof structurer; Single-band fluorescence cubes:
      • (EF: Exciter filter, BS: Beam splitter, BF: Barrier filter)
      • Red: EF: 527 - 552 nm, BS: 565 nm, BF: 577 - 632 nm
      • Green: EF: 465 - 495 nm, BS: 505 nm, BF: 515 - 555 nm
      • Blue: EF: 360-370 nm, BS: 410 nm, BF: 420-460 nm
    • Fluorescence illuminator: Straight design with field iris diaphragm
      pEGFP-UHRF1 and pmCherry-TRIM21 (2 plasmids) coinjected into CHO-K1 cells
      pEGFP-UHRF1 and pmCherry-TRIM21 (2 plasmids) coinjected into CHO-K1 cells
  • Compatible with standard cell culture well plates
    The large XY-stage of the system can host standard well plates of the major brands, with up to 24 wells.
  • Cell-friendly environment
    Perform experiments within cell culture context, without perturbing it. Fully enclosing incubator with temperature control and a HEPA-Filter system, custom-designed around the CRISPR system.

    Specifications:

    • Black polycarbonate casing for optimal fluorescence imaging
    • Integrated, high precision temperature control system
    • Integrated airflow control system
    • Long-lasting, high Efficiency Particulate Air (HEPA) filter
    • 2 x 10W UV decontamination lamps with automatic life cycle monitoring & time control
    • 4 x white LED light source
    • Tailor-made doors for easy access to main CRISPR system and microscope areas
    • Power consumption 620 W

    Extra feature: CO2 controlled environment (coming soon)
    When a CO2-independent medium is not a viable option, control of the CO2 concentration in the atmosphere is crucial. Then, it is advisable to equip the incubator with CO2-control capabilities.
    Incubator V3.6 without camera

  • Proven injection protocols
    Access Cytosurge's database of proven injection protocols, see here.
  • Observe & follow up
    Cytosurge's software records the XY-coordinates of the edited cells and allows the user long-term observation of the modified cells with the desired settings (fluorescence filter, exposure time etc...). Observe any point of interest and follow how events unfold with multi-channel time-lapses.
  • Save on material costs
    Only 1µl of CRISPR-Cas solution is required to inject thousands of cells.
Software

Point-and-click

According to the high definition image of the cells on the screen, chose the cells of interest according to their phenotype. Select hundreds of cells under visual control, selectively target for the nucleus or cytoplasm.

Easy walkthrough procedures

  • Follow the in-software suggested instructions to optimally deliver your CRISPR-Cas complexes into the nucleus

  • Apply recommended injection settings from our protocols database

  • Easily tailor injection parameters to your cells by changing few parameters (injection pressure, injection time, exerted force and approach speed)

  • Finally, step back and let the CRISPR system autonomously deliver the CRISPR-Cas complexes

Follow up your experiment

Instruct the system to take the needed images with your favorite settings, in few clicks. See all relevant data grouped by the coordinates of each cell.

Volume Calculator – integrated feature

Simply select the images of the modified cells and follow the on-screen step-by-step instructions to obtain the real injected volume.

Repeatability

Find the modified or chosen cells at any time, and even repeat injection into a same cell.

Chose the cells of interest according to their phenotype
Follow the in-software suggested instructions
Instruct the system to take the needed images with your favorite settings
Follow the on-screen step-by-step instructions to obtain the real injected volume
Find a cell and repeat injection if needed
CONSUMABLE - FluidFM nanosyringe

Developed for injection into single cells: the very sharp apex and the around 600 nm aperture at the pyramidal tip of the FluidFM nanosyringe enable gentle injection not compromising cell viability and allow to work with compounds with several molecular weights and densities. The FluidFM nanosyringe can be loaded with any liquid with viscosities from 1 to 10000 cP (water to honey).

  • Various cell lines – ideal for adherent mammalian cells like CHO, HeLa, HEK

  • Reliable –  inject into thousands of cells with the same nanosyringe

  • Easy handling – automatic consumable exchange and washing features

  • Gain ultimate insights – know the real shape of each nanosyringe in use by inspecting the SEM picture downloadable with the QR-Code printed on the blister

  • 1µl reservoir – reduce the amount of reagent: only 1µl of CRISPR-Cas solution is required to inject into thousands of cells

Specifications:

  • Aperture diameter: 600 nm

  • Tip & aperture shape: Pyramidal side aperture

  • Available spring constants: 2 N/m

  • Probe dimensions: Length 200 µm, Width 36 µm, Height 1 - 2.5 µm, Pyramid height 7 µm

The pyramidal tip of the FluidFM nanosyringe enable gentle injection not compromising cell viability
FluidFM nanosyringe aperture
Specifications and real shape of the used FluidFM nanosyringe known by scanning the QR-code on the package