The qEV10 / 35 nm is now available in the Gen 2 range. Gen 2 qEV columns are built with Izon's new proprietary, agarose resin which delivers a more purified extracellular vesicle (EV)-containing eluate. The qEV10 / 35 nm Gen 2 column is designed for separating EVs from a 10 mL biological sample and comes with Luer Lock fittings, which allow for many different configurations.
The qEV10 / 35 nm Gen 2 has an optimal recovery range of 35 nm to 350 nm and is compatible with the Automatic Fraction Collector (AFC). AFCs are used to adjust the purified collection volume (PCV) to provide reproducible isolation for downstream analysis.
Izon products are designed and manufactured under a quality system certified to ISO 13485:2016.
qEV10 Gen 2 is also available in the 70 nm series.
Discounts are available on bulk orders of qEV Isolation Columns. Please contact us to find out more.
Product Code: IC10-35
Files
| Attachment | Size |
|---|---|
 qEV10 Gen 2 Quick Start Guide | 84.84 KB |
| qEV10 Gen 2 User Manual | 1.7 MB |
Technical Features
Optimal Separation Size | <110nm |
Buffer Volume * | 23,2 mL |
Sample load volume | 10 mL* |
Column Volume | 69,3 mL |
Optimal fraction size | 5 mL |
Flush Volume | 140 mL |
PCV* | 20 mL |
Elution peak after buffer volume* | 15 ± 5 mL |
Operational temperature | 18 to 24°C |
Buffer | PBS |
Upper, lower and loading frit size | 20 µm |
pH stability working range | 3-13 |
pH stability cleaning-in-place (CIP) | 2-14 |
Shelf life | 12 months (if stored correctly) |
*Loading higher sample volumes results in a lower level of purity in the later vesicle volumes, greater overlap between protein and EV elution peaks, and a higher protein peak within the PCV. Loading lower sample volumes results in a higher dilution factor of the sample. The optimal recommended sample volume for purity on the qEV10 Gen 2 is 10 mL.
**Values based off analysis of human plasma samples.