Size range: 35nm - 350nm - SEC Columns for Exosomes Separation and Purification
Also available in the 70nm+ series, this new 35nm+ column has an optimal recovery range of 35nm to 350nm.
Izon's most popular qEV column, the qEVoriginal / 35nm contains a resin with an approximately 35 nm pore size. Proteins and other contaminating molecules smaller than 35nm enter the pores of the resin and are delayed in their passage through the column, eluting in later fractions. The use of qEV columns leads to higher quality Exosome measurements and analysis when combined with TRPS measurements.
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Under ideal conditions, SEC purification of vesicles is able to remove >99% of contaminating free protein
A significant problem with ultracentrifugation is that the similar density of HDL and LDL particles to Exosomes means that the particles often co-isolate with Exosomes. The collection of appropriate fractions from qEVoriginal separation results in up to 95% removal of contaminating HDL from Exosome samples.
qEVoriginal columns have undergone extensive research and development to ensure that the separation does not introduce any bias into the sample.
qEVoriginal columns may be used up to 5 times, with a cleaning step in between uses, making their use very cost effective compared to all other separation options.
qEVoriginals are presently 10 ml volume, targeted at 500 μL of loading volume.
All of Izon's qEV columns are certified to ISO 13485, the quality assurance standard for medical devices, which is an essential requirement.
Comparison with other Techniques
|Method||Purity||Exosome yield||Protein Yield||Ease of use||Turn around Time (hrs)||Hands On time (hrs)||Cost per test (USD)|
|Legend *=low, **=moderate, ***=high ****=very high|
 Dependant on reuse