Capable of running 100 mL samples, the qEV100 is ideal for any application that requires large sample volumes, whether it’s the output of bioreactors, dilute cell culture supernatants or materials with high protein levels like plasma or serum. Many of these biological fluids cannot be concentrated further due to the propensity for protein complex formation and vesicle aggregation, which can destroy biological function. Like all of Izon’s qEV columns, the qEV100 provides a gentle, rapid method for maximizing recovery of biological particles. No ultracentrifugation. No organic solvents.
The qEV100 is also available with the 70 nm+ Isolation range
Videos
Files
Attachment | Size |
---|---|
qEV100 User Manual | 590.77 KB |
qEv Overview Brochure | 2.67 MB |
Summary of qEV recommendation and sample preparation by sample type | 680.87 KB |
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Technical Features
Column Name | qEV100 | qEV100 |
Column series | qEV100 / 70 | qEV100 / 35 |
Optimal Separation Size | 70 - 1000 nm | 35 - 350 nm |
Nominal flow rate (mL/min at 20°C) | 15 | 11 |
Column Volume (mL) | 750 | 750 |
Sample Load Volume (mL) | Up to 100mL | Up to 100mL |
Optimal fraction size (mL) | 25 or 50 | 25 or 50 |
Void volume (mL) | 150 | 150 |
Minimum flush volume (mL) | 700 | 700 |
Nominal peak elution volume (mL) | 200 | 200 |
Elution peak after void (mL) | 75 ± 25 | 75 ± 25 |
Operational temperature | 18 to 24 °C | 18 to 24 °C |
Buffer | PBS | PBS |
Largest size passable | 1 µm | 1 µm |
Top and bottom filters size | 20 µm | 20 µm |
pH stability working range | 3 - 13 | 3 - 13 |
pH stability cleaning-in-range (CIP) | 2 - 14 | 2 - 14 |
Shelf life (if stored correctly) | 12 months | 12 months |