A clean and efficient system for concentrating isolated EVs. The qEV Magnetic Concentration Kit contains Magnetic Nanotrap® Extracellular Vesicle Particles, manufactured by Ceres Nanosciences for Izon Science. Simply add the specified volume of Nanotrap® particles to the EV sample and incubate at room temperature for 10 minutes. After applying a strong magnet for 2 minutes, the supernatant can be removed to obtain the pellet containing the EVs. This rapid and clean EV concentration method eliminates the need for centrifugation.
The kit utilizes Magnetic Nanotrap® Extracellular Vesicle Particles from Ceres Nanosciences: chemically functionalized hydrogel polymers surrounding a magnetic core. The kit captures and concentrates EVs, which can then be used for downstream analyses such as Western Blot, PCR, or RNA studies.
The binding of the particles is irreversible, an important factor for downstream applications.
Product Code: RCT03
Contents: 5 mL of Magnetic Nanotrap® EV Capture Particles.
Videos
Files
| Attachment | Size |
|---|---|
 qEV Magnetic Concentration Kit bROCHURE | 391.84 KB |
| qEV Magnetic Concentration Kit Quick Start Guide | 65.73 KB |
| qEV Magnetic Concentration Kit User Manual | 510.21 KB |
Technical Features
PARTICLE TYPE | FORMAT |
|---|---|
Nanotrap® EV Capture Particles | Magnetic Nanotrap ® Particles |
qEV COLUMN | INPUT qEV VOLUME | OUTPUT VOLUME (DEFAULT PCV)* |
|---|---|---|
qEVsingle Gen 2 | 150 uL | 680 uL |
qEVoriginal Gen 2 | 0.5 mL | 1.6 mL |
qEV1 (Gen 2 only) | 1 mL | 2.8 mL |
qEVsingle Legacy | 150 uL | 800 uL |
qEVoriginal Legacy | 0.5 mL | 2.0 mL |
qEV COLUMN USED FOR | PURIFIED COLLECTION | VOLUME OF NANOTRAP^{®} EV |
|---|---|---|
qEVsingle Gen 2 | 0.51-0.85 mL | 20 uL |
qEVoriginal Gen 2 | 1.2-2.0 mL | 40 uL |
qEV1 (Gen 2 only) | 2.1-3.5 mL | 70 uL |
qEVsingle Legacy | 0.60-0.80 mL | 20 uL |
qEVoriginal Legacy | 1.5-2.0 mL | 40 uL |
*The Default Purified Collection Volume is the default Purified Collection Volume (PCV) value specific to each qEV column, selected to provide a balance of high EV recovery and purity. It is programmed on the Automatic Fraction Collector and can be adjusted; refer to your qEV user manual for more information.