Exicycler™ 96 Real-Time Quantitative Thermal Block is a self-patented 96-well simultaneous detection technology using LT (Light Tunnel) technology that emits uniform excitation light,excitation light blocking method using polarized light, and 2-D sensor. And it is a real-time gene amplification instrument that minimized optical sensitivity and deviation between wells. The thermal block is designed in a low-profile 96-well format, minimizing the volume of the solution used in the PCR reaction and reducing the PCR reaction time.
Files
| Attachment | Size |
|---|---|
 PM-727-En(V2) Exicycler96V4Fast_20161208.pdf | 4.65 MB |
Bioneer patented fluorescence detection method (Korean Patent No. 10-1089045, US Patent No. US 8427643) was applied to minimize noise caused by reflected light, thus improving sensitivity and accuracy.
Figure. Separation of a reflection light from fluorescence
* Noise caused by reflected light: The fluorescent materials used in qPCR create emission when the absorbed excitation energy excites them then falls to the ground state. In conventional qPCR instruments, part of excited light irradiated from the sample is reflected from the surface of the plate and the instrument, which may enter the detector to be also mistakenly read as part of the light from the sample. As the excited light has much more luminance than the fluorescent light, reflected light also contain high luminance, making it unable to be completely removed by the fluorescent light and creating noise in the process, deteriorating sensitivity and accuracy. However, by using our patented technology, excitation and fluorescence have different polarity, allowing the reflect light unable to be blocked by fluorescent filter to be filtered once again through polarizing filter. This allows drastic reduction of noise caused by reflected light compared with other qPCR instruments.
Features and Benefits
- High reproducibility and accuracy
Highly reproducible and accurate results by light tunnel (LT) technology (Korean Patent No. KR 0794703, US Patent No. 8139210, Japanese Patent JP 4751821, China Patent No. CN 1798969) irradiating uniform excitation light, excitation light interception method (Korean Patent No. 10-1089045 and US Pat. No. 8427643) using polarization, and a 96-well simultaneous detection technique using a 2-D sensor minimizing optical sensitivity and deviation between well
- Reduce reaction time
Maximum ramping rate of 4.5 ℃/sec (Exicycler ™ 96 Fast: max 5.0 ℃/sec) decreases reaction time by using a unique thermal block and temperature control technology
- 5 color multiplexing system
Simultaneous detection of five targets capable of being used in multiplex PCR without the need of reference dyes, used for the compensation of light quantity deviation, through the uniform optical system patent technology (Korean Patent KR 0794703, US Patent US 8139210, Japanese Patent JP 4751821, China Patent CN 1798969)
- Convenience
Diverse choice of fluorescent dyes including the universal ones with wide excitation/emission wavelength detection range being 475 to 690nm, unlike other traditional instruments designed for specific dyes
- Wide dynamic range
Large detection range being more than 9 log allowing DNA detection and qualitative analysis even at low and high concentration samples using a short Arc lamp, a powerful excitation light source
- Temperature gradient function
Simplified search of optimal PCR annealing temperature with temperature gradients from 1~20°C available in the range of 20~95°C
- Various application
Wide applications such as touch down PCR through time increment and temperature increment adjustment functions
- User-friendly software
Accessible User-centric GUI for easy operation and manipulation for designing experiments and analyzing results
Technical Features
| Dimension (cm) | 35.5 (W) x 54 (D) x 47 (H) |
| Weight (kg) | 39 |
| Sample capacity/size | Fast block: Opaque White 96-well Low Profile PCR Plate / 0.1 ml Opaque White 8-strip Low Profile PCR Tube |
| Normal block: Opaque White 96-well PCR Plate /0.2 ml Opaque White 8-strip PCR Tube |
|
| Sample volume | Fast block : 10 ~ 50 ㎕ |
| Normal block : 20~100 ㎕ (50 ㎕ recommended) | |
| Power consumption | 100 - 240 VAC, 50/60 Hz, Max 800 VA |
| Operating temperature | 15 ~ 35℃ |
| Operating humidity | 20 ~ 80%, no condensation |
| Thermo module specifications | |
| Method of heating/cooling | Peltier |
| Temperature range | 4.0°C ~ 99.9°C |
| Max ramp rate | Fast block : 5.0°C/sec |
| Normal block : 4.5°C/sec | |
| Temperature accuracy | ± 0.3°C |
| Temperature uniformity | ± 0.3°C |
| Lid temperature | 90 ~ 120°C |
| Temperature increment range | 0.1°C ~ 2.0°C |
| Time increment range | 1 sec ~ 60 sec |
| Gradient range | 20°C ~ 95°C |
| Ramp rate control | 1~100% |
| Computer specifications | |
| Operating system | Window 7 & 10 (32/64 bit OS) |
| Processor speed | Intel Dual Core E2160 (1.8GHz) or higher |
| Memory | 1GB or higher |
| Communication port | USB 2.0 high speed |
| Screen resolution | 1280X1024 or higher |
| Optical specifications | |
| Light source | Short arc lamp (120W) |
| Sensor | 16 bit 2D CCD |
| Excitation filter/emission filter | 5 sets* |
※Specifications of this product are subject to change without notice to improve product performance.
Filter sets
| Position | Excitation (㎚) | Emission (㎚) | Set | Applicable Fluorescent dye |
| 1 | Blank | Blank | 1 | - |
| 2 | 475 | 530 | 1 | FAM, SYBR Green I |
| 3 | 520 | 560 | 1 | JOE, TET |
| 4 | 550 | 590 | 1 | TAMRA, CY3 |
| 5 | 570 | 630 | 1 | Texas Red, ROX |
| 6 | 630 | 690 | 1 | CY5 |