The qEV100 Gen 2 is the largest column in the qEV range, optimised for a sample loading volume of 100 mL. The qEV100 Gen 2 is ideal for research and applications involving large sample volumes, including cell culture supernatant. Like all columns in the qEV range, the qEV100 Gen 2 provides a gentle, rapid method for maximising the separation of EVs from soluble protein.
The qEV100 / 70 nm Gen 2 has an optimal recovery range of 70 nm to 1000 nm.
qEV100 Gen 2 is also available in the 35 nm series.
Note that the qEV100 Gen 2 is not compatible with the Automatic Fraction Collector (AFC).
For more information and to compare qEV columns, please visit the qEV Isolation Overview.
Discounts are available on bulk orders of qEV Isolation Columns. Please contact us to find out more.
Izon products are designed and manufactured under a quality system certified to ISO 13485:2016.
Product Code: IC100-70
Videos
Files
| Attachment | Size |
|---|---|
 qEV100 SEC User Manual | 417.52 KB |
| qEv Overview Brochure | 2.67 MB |
| Summary of qEV recommendation and sample preparation by sample type | 680.87 KB |
Technical Features
Column Name | qEV100 | qEV100 |
Column series | qEV100 / 70 | qEV100 / 35 |
Optimal Separation Size | 70 - 1000 nm | 35 - 350 nm |
Nominal flow rate (mL/min at 20°C) | 15 | 11 |
Column Volume (mL) | 750 | 750 |
Sample Load Volume (mL) | Up to 100mL | Up to 100mL |
Optimal fraction size (mL) | 25 or 50 | 25 or 50 |
Void volume (mL) | 150 | 150 |
Minimum flush volume (mL) | 700 | 700 |
Nominal peak elution volume (mL) | 200 | 200 |
Elution peak after void (mL) | 75 ± 25 | 75 ± 25 |
Operational temperature | 18 to 24 °C | 18 to 24 °C |
Buffer | PBS | PBS |
Largest size passable | 1 µm | 1 µm |
Top and bottom filters size | 20 µm | 20 µm |
pH stability working range | 3 - 13 | 3 - 13 |
pH stability cleaning-in-range (CIP) | 2 - 14 | 2 - 14 |
Shelf life (if stored correctly) | 12 months | 12 months |