ExiCycler™ V5 is an innovative real-time PCR with Bioneer’s patented technology, providing researchers a fast and accurate results. Through a maximum 10.0℃/sec ramping rate, it minimizes experimental time and supports researchers in achieving highly efficient and accurate experiments.
Features & Benefits
- Rapid and Precise Results
Real-time PCR experiments now offer faster and more accurate results than ever before
- Patented thermal block technology allows for ramping rates of up to 10.0℃/sec
- With a running time of just 40 minutes, a multitude of experiments can be conducted within a short timeframe
- Light Polarization Technology ensures exceptional sensitivity and accuracy
- Intuitive User Interface (UI)
Easy operation with a 10-inch touchscreen and user-friendly interface
- Operate Independently without a PC
- Monitor protocol and view result graphs in real time
- Easily access past experimental files during the experiment (Open Data File Technology)
- 6-Channel Multiplexing
True 6-channel Multiplex without the need to use reference dye
- Alongside FAM, TET, TAMRA, Texas Red, and Cyanine5, the V5 model includes ATTO 425, expanding compatibility to 10 fluorescent dyes with 6 filters.
- Wide Excitation/Emission detection range (430nm ~ 690nm)
- Able to test for a variety of pathogens using one sample
- Convenience
We assist researchers in efficiently conducting experiments
- Offer two types of thermal blocks that are suitable for various experimental purposes (fast/normal)
- Both 0.1ml and 0.2ml tubes are compatible
- Establish optimal PCR conditions using the thermal gradient function
- Supports analysis software suitable for diverse experimental fields
- Ensure stable performance and long lifespan by using high-performance LED
Files
| Attachment | Size |
|---|---|
 PM-ExiCycler_V5_RUO-EN.pdf | 3.63 MB |
| ISO13485(instrument)_2024.pdf | 437.63 KB |
Our patented nano alloy block and temperature control technology enable ramping rates of up to 10℃/sec, reducing PCR time to just 40 minutes.
We offer gradient functions, time and temperature increment options, and ramping rate control for diverse customer applications.
Users can select between 96-well, 96-well fast, 384-well, and 384-well fast formats, allowing selection of 4 types of thermal blocks for desired speed and number of samples.
For added convenience, we've included a 10-inch touchscreen and intuitive User Interface(UI).
Easily control PCR Protocol settings, and view graphs—all without a PC.
ExiCycler™ V5 minimizes optical sensitivity and well deviations with its patented excitation light blocking technology using polarized light and
using polarization and simultaneous detection with a high-sensitivity CMOS camera.
This enables 6-channel multiplex qPCR without the need for reference dye, improving understanding of experiment reagents and reducing consumption.
Technical Features
| Physical Specifications | |
| Dimension (W x D x H) | 32 cm x 51.5 cm x 44.5 cm (12.6 in x 20.3 in x 17.5 in) |
| Weight (kg) | 29.2 kg (64.4lbs) |
| Sample Capacity / Size | 96-well, 384-well |
| Sample volume | 96-well 0.1 ml tube/plate: 10 ~ 50 ㎕ |
| 96-well 0.2 ml tube/plate: 20 ~ 50 ㎕ | |
| 384-well 0.02ml plate: 5 ~ 20 ㎕ | |
| Voltage | 100 - 240 V~, 50/60 Hz |
| Power consumption | 800 VA max (Fuse 250 V 10 A) |
| Environmental Specifications | |
| Operating temperature | 15 - 35 °C (59-95 ℉) |
| Operating humidity | 20 - 80 % (relative humidity, no-condensation) |
| Transport and storage temperature | 0 - 40 °C (32 - 104 ℉), in shipping container |
| Transport and storage humidity | 20 - 80 %, no condensation |
| Over voltage category | Over voltage category: II |
| Software Specifications | |
| Software version | 5.10.xx |
| Operating Specifications | |
| Operating system | Built-in (window 10 IoT) |
| Networking | TCP/IP, USB |
| User interface display | 10.1 inch touch screen LCD |
| Data storage | 128 GByte |
| Environmental Specifications | |
| Method of heating and cooling | Peltier |
| Temperature range | 4.0 - 99.9 °C |
| Max heating rate | ExiCycler™ V5 96: 8.0 °C/sec |
| ExiCycler™ V5 96 Fast: 10.0 °C/sec | |
| ExiCycler™ V5 384: 5.5 °C/sec | |
| ExiCycler™ V5 384 Fast: 7.0 °C/sec | |
| Temperature accuracy | ± 0.2 ℃ |
| Temperature uniformity | Normal: ≤ 0.2 ℃, Fast: ≤ 0.3 ℃ |
| Lid temperature | 90 - 120 ℃ |
| Gradient range | 30 - 95 ℃ |
| Temperature differential range | 1 - 20 ℃ |
| Temperature increment range | 0.1 ~ 2.0 ℃ |
| Time increment range | 1 - 60 sec |
| Ramp rate control range | 1 - 100 % |
| Operating Specifications | |
| Light source | White LED |
| Sensor | CMOS |
| Excitation Filter / Emission Filter | 6 Sets |
※Specifications of this product are subject to change without notice to improve product performance.
Filter sets
| Position | Excitation (㎚) | Emission (㎚) | Set | Fluorescent dye |
| 0 | 430 | 480 | 1 | ATTO425 |
| 1 | 470 | 520 | 1 | FAM, SYBR Green I |
| 2 | 515 | 560 | 1 | JOE, TET |
| 3 | 550 | 585 | 1 | TAMRA, Cyanine3 |
| 4 | 570 | 615 | 1 | Texas Red, ROX |
| 5 | 630 | 690 | 1 | Cyanine5 |