ExiProgen™ is an instrument that automatically synthesizes protein from DNA. This product performs In-vitro transcription and translation automatically when loaded with DNA coding a protein and a protein synthesis kit. By purifying this protein through affinity chromatography method, more than 90% pure protein can be obtained within 6 hours and up to 16 species can be produced at one time. In addition, highly expressed affinity tagged proteins can be purified in cell lines with high purity.
Features and Benefits
- Built-in optimized nucleic acid and protein synthesis protocol
Simplified usage and reproducible results with built-in nucleic acid extraction and protein purification protocols optimized for various samples (whole blood, tissue, cell, bacteria, plant, etc.)
- High stability
Minimized protein denaturation by installing cooling reaction block inside the instrument keeping the temperature of elution tube rack below 10°C
- High-pure nucleic acid extraction & protein purification
Quick performance of nucleic acid extraction and protein purification with great purity and efficiency by attaching magnetic particles to the bottom of each well equally and rapidly by magnetic blocks attached to the instrument
Increased elution efficiency allowing complete evaporation of alcohols by its heating block (Korean Patent No. 10-1025135) - Contamination prevention device
Substantial blocking of cross-contamination, which may occur in other wells by the aerosols generated from the tip, through the sophisticated designs drop-contamination prevention device located at the bottom of the filter tip
- Automatic UV sterilization Function
Sterilization to prevent infection for the users by the samples through the UV lamp installed inside the instrument
- Easy-to-use
User-friendly UI capable of checking the experimental progress rate real-time without the need of seperate PC by equipping with a 13.3-inch touch screen on the front of the device
- Excellent precision
Minimized error range, that may occur during the automatic dispensing of the solution, through installment of an adjustable syringe motor up to 0.1 mm capable of stably distributing in a volume of 25 ㎕ ~ 1000 ㎕ for application to various protocols and obtaining of experimental results
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| 3.12 MB | |
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Protein synthesis using ExiProgen™ is carried out using its compatible ExiProgen™ Protein Synthesis Kit Series. ExiProgen™ performs protein expression using Cell-free Protein Synthesis system, and affinity purification using Ni-NTA magnetic beads, both in a fully-automated way. After adding a target gene to the template DNA sample and running the instrument, the protein is expressed in 3 hours with the help of Cell-free Protein Expression reagent. The expressed protein is then collected using Ni-NTA magnetic bead, and is purified using a reagent to yield a final high-purity target proteins.
Technical Features
| Dimensions (cm) | 32 (W) x 53.5 (D) x 50 (H) |
| Weight (kg) | 27 |
| Temperature range | 15-35℃ |
| Humidity range | 20-80%, no condensation |
| Operating system | Standalone |
| User interface | 320 x 240 touch screen graphic LCD |
| Input voltage | 100 – 240 VAC |
| Frequency | 50 / 60 Hz |
| UV sterilization | 15 minute cycle |
| Communications | TCP/IP |
| Heat block | 40-90℃ |
Function
| DNA/RNA Extraction | O |
| Protein Expression | O |
| Protein Purification | O |
| TFT Touch Screen | O |
| Sample Number | 16 samples |
| UV sterilization | O |
| Heating & magnetic block | O |
| Cartridge Contamination shield | O |
| TCP/IP network connection | Numerous equipment can be connected |
| Cooling system for sample store | O |
| Automatic dispensing | O |
| Power supply | 24 VDC, 7.5 A(180 W) |


