Seeing LLPS (liquid liquid phase separation) with holotomography

Many researchers were interested in seeing LLPS (liquid liquid phase separation) with holotomography.
We introduce LLPS observed by Tomocube's HT in a publication by Choi et. al. - "Aggresomal sequestration and STUB1-mediated ubiquitylation during mammalian proteaphagy of inhibited proteasomes."
The paper shows that inhibited proteasomes (by treating with MG132) are actively sequestered into the aggresome and colocalized with the autophagic receptor SQSTM1. Visualized with Tomocube's HT, proteasome-inhibited cells showed juxtanuclear inclusion bodies accumulating with an exceptionally high refractive index, concentration, and sphericity compared to the control group, . The localization of these high RI aggregates were confirmed to be surrounded by SQSTM1-positive inclusion bodies as seen by HT-2H correlative fluorescence imaging.