Full CellSorter Instrument Including Fluorescent Microscope
Brand:
- High throughput single cell sorting directly from the Petri dish
- One single cell arrives to each PCR tube
- 10 PCR strips containing 80 tubes can be filled in a cycle
- Glass cover slip for testing single cell deposition in situ
- Drop volume less than 1 ul for adherent cells
- Pick up volume of ~1 nl for suspended cells
- 15-20 seconds per cell. When collecting multiple cells, sorting speed is 1 cell/second.
- Number of cells picked up in a single run: 1-1000.
- Isolates a subpopulation of live adherent cells expressing fluorescent or luminescent markers
- Both unlabeled and fluorescentcells are recognized by computer vision
- Viable cells after sorting
- Any adherent and non-adherent cell type can be sorted
- Cell culture needs minimal preparation before sorting
- Average sorting process takes only a few minutes
- Multichannel detection using the fluorescent filter setup of the microscope
Components
- Valve Controlled Cellsorter Device & Software
- High precision micropipette holding console made from hard aluminum equipped with LED illumination for micropipette focusing connected to the motorized micromanipulator via a rotatable arm.
- 25 mm travel range 1D motorized micromanipulator
- Special aluminum insert fitting to the microscope stage with a hole and magnetic fixing ring for the 35 mm Petri dish and 80 holes for 10 PCR stripes and a hole for a 32x24 mm2 glass cover slide for testing and high resolution imaging and a hole with a calibration cross-hair
- CellSorter control unit with 2 high speed fluid valves and USB connection to the computer
- User friendly intuitive control software for MS Windows OS
- Accessories, fittings and tubing for liquid control
- 5 pcs of borosilicate micropipettes for single cell manipulations with calibrated aperture
- Motorized Zeiss Axio Observer A1 Inverted Fluorescent Microscope
- Including NA 0.55 LD condensor lens, EC Plan neofluar 10x/0.3 Ph 1 objective lens
- Accessories for phase contrast and fluorescent imaging
- Fluorescent illumination system with Prior Lumen 200S metal halide light source
- Marzhauser 2D motorized microscope stage and motorized focus drive.
- Andor Zyla 5.5 Camera
- USB 3.0 sCMOS – Compact low light imaging camera
- Front-illuminated scientific CMOS sensor
- 2560 x 2160 pixel array, 6.5 μm pixel size
- Low read noise of 1.2 e-, sensor stably cooled to 0°C up to an ambient temperature of +35°C
- Rolling shutter and ‘true’ global shutter modes
- 12 bit and 16 bit digitization
- Max. pixel readout rate 2x280MHz
- iCam, C-mount, USB3.0 interface (interface card and cable included), power supply, trigger cable
- NE-1000 Syringe Pump
- Holds 1 Syringe up to 60 cc
- Infusion rates from 0.73 µL/hr (1 cc syringe) to 2100 ml/hr (60 cc syringe)
- Easy-to-use keypad interface
- Space Saving Chassis: Foot print size of only 5 3/4" x 8 3/4"
- Includes all the advanced functions and programming features of the NE-1000 family
- Dell Desktop Computer With 23" Dell Monitor
- Processor: Intel Core i7-4790 3,6: 4 Cores, 8M Cache, up to 4.00 GHz
- Memory: 8 GB DDR RAM
- HDD: 1000 GB
- SSD: 120 GB